flowAI report
SIgN_GM
FCS file information
Input file name: Donor_EDTA-Blood_031
Number of events: 476717
Quality control analysis
Summary
The anomalies were removed from: Flow Rate, Flow Signal and Flow Margin
Anomalies detected in total: 42.17 %
Number of high quality events: 275705
Flow rate check
24.72 % anomalies detected in the flow rate check.
The plot reconstructs the flow rate with a resolution of 1/10 of a second. Anomalies in the flow rate are identified with an algorithm based on the generalied ESD outlier detection method. The anomalies are circled in green.
Signals acquisition check
34.76 % anomalies detected in the signal aquisition check.
The more stable region selected consistent for all channels is highlighted in yellow. If the removal of outliers has been required before the execution of the changepoint analysis, the detected outliers are cicled in green.
The FCS file was divided in 954 bins, hence each bin contains 500 event(s). The stable region is located between the bins 1 and 622.
More info on the changepoints detected
Changepoints detected in channels with shifts in signal acquisition:
## 1
## 488 715/30-A 632
## 405 515/20-A 622
## 561 585/15-A 626
## 561 610/20-A 644Channels excluded from the analysis: FSC-A, FSC-W, SSC-A, SSC-W.
No changepoints were detected in the channels: 488 525/50-A, 405 450/50-A, 405 605/40-A, 405 670/30-A, 405 710/40-A, 405 780/60-A, 355 379/28-A, 355 515/30-A, 355 560/40-A, 355 740/35-A, 355 820/60-A, 640 670/30-A, 640 730/35-A, 640 780/60-A, 561 780/60-A, 405 585/15-A.
Dynamic range check
1.13 % anomalies detected in dynamic range check.
The plot shows where the anomalies occured the most. The x-axis scale is complementary to the one of the signal acquisition plot.
More info on the anomalies detected in the dynamic range check
For each channel analyzed, the table shows the number of events that did not pass the dynamic range check:
## lower_range upper_range
## 488 525/50-A 401 1057
## 488 715/30-A 140 11
## 405 450/50-A 121 8
## 405 515/20-A 57 852
## 405 605/40-A 13 53
## 405 670/30-A 111 27
## 405 710/40-A 136 11
## 405 780/60-A 240 9
## 355 379/28-A 220 99
## 355 515/30-A 30 8
## 355 560/40-A 111 71
## 355 740/35-A 147 44
## 355 820/60-A 203 7
## 640 670/30-A 138 8
## 640 730/35-A 177 8
## 640 780/60-A 225 12
## 561 585/15-A 110 3508
## 561 610/20-A 80 1236
## 561 780/60-A 146 18
## 405 585/15-A 24 41
## total_SUM 2830 7088
## total_UNIQUE 593 4784Channels excluded from the analysis: FSC-A, FSC-W, SSC-A, SSC-W.